barSeed - Barley Seed Development Database

Probe CUST_14089_PI390587928

Source Contig U35_15702

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_14089_PI390587928	Hv_44K_v2	CGTGTGATGATGGAACCTCATTTCCTGGAAAATAATTCAGAGTGTTTTGGACGCAAAAAA	1738	GeneList: brown MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.823	2.001	1.158	0.853	0.366	0.423
Raw	9099.817	15387.517	7891.570	9269.823	1368.603	1722.640

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_79988.1	1711	3e-21	1658 - 1711	100	54 / 54	plus	ABC03640
JLOC1	JLOC1_53263.1	2282	5e-22	1927 - 1981	100	55 / 55	plus
Harvest21	ABC03640	1855	3e-25	1739 - 1798	100	60 / 60	plus	ABC03640
Harvest35	U35_15702	1856	3e-25	1739 - 1798	100	60 / 60	plus

Contig Sequence (1856 bp)

>U35_15702 1856 Hv_44K_v2
GGTTCGCGGTGGTCGGGCTCCCCGCGGCGGCGAGGAGGGGGAGGCGGCGCGGCGGGGCCG
TGGCGGCCAGCCCACCCACGGAGGAGGCGGCCAAGCTGACGGAGCCGCTCACCAAGGAGG
ACCTCGTCGCCTACCTCGCGTCCGGGTGCAAGCCCAAGGAGAACTGGAGAATTGGCACAG
AACATGAGAAGTTTGGTTTTGATGTCAAAACATTGCGCCCTATAACGTATGATCAGATCA
GTGCCATACTCAATGGGCTGTCTGAGAGATTTGAATGGGACAAGATAATGGAAGAAAACC
ATGTTATTGGTCTCAAGCAGGGAAAGCAAAACATTTCACTAGAACCTGGTGGCCAGTTTG
AACTTAGTGGCGCTCCCCTTGAAACATTACATCAAACTTGTGCGGAGGTCAATTCACATC
TTTATCAGGTCAAAGCAGTTGGAGAGGAATTGGGAGTAGGATTTCTTGGAATGGGTTTTC
AGCCAAAATGGGCCCTAGCTGATATACCAATAATGCCCAAGGGAAGGTATGAAATTATGA
GGAATTACATGCCTAAAGTTGGTACTCTTGGCCTTGATATGATGTTCCGCACGTGCACTG
TACAGGTTAATCTCGATTTCAGTTCTGAGCAAGATATGATAAGAAAATTCCGTGCTAGCC
TTGCATTGCAACCTATCGCGACAGCAATATTCGCAAATTCTCCCTTCAAAGAAGGAAAAC
CAAATGGGTTTCTCAGTTTAAGAAGCCATATCTGGACTGATACTGACAACAACCGCTCGG
GGATGCTTCCTTTTGTTTTTGATGACACATTCGGATTTGAGCAATATGTGGACTATGCAT
TGGACGTACCAATGTATTTTGTATACCGGAACAAGAATTACATTGACTGTACTGGGATGT
CATTTCGGGATTTTATGGCAGGAAAGCTCCCACAGGTTCCAGGGGAGTTGCCCACTTTGA
ACGATTGGGAGAACCATCTAACAACAATTTTTCCTGAGGTTAGGTTGAAGAGATACATGG
AGATGAGAGGTGCTGATGGTGGACCTTGGAGGAAATTGTGTGCTTTGCCTGCATTTTGGG
TTGGGTTGTTGTATGACGACGAGTCACTACAGAGCATTATTGACATGACTTCTGACTGGA
CAAAGGAAGAGAGAGAGATGTTGAGACGGAAGGTGCCAGTTACTGGTTTGAAGACACCAT
TCCGGGATGGGTATGTAAGAGATTTAGCTGAAGATGTTCTCCAGTTAGCTAAGAATGGAC
TAGAAAGAAGAGGGTACAAGGAGGTTGGTTTCCTGAGAGAGGTCGATGAAGTTGTTAGAA
CAGGAGTGACACCTGCTGAGAAGCTTCTGAATCTATACGAGACGAAGTGGCAGCGCAGCG
TGGACCCCGTCTTCGAGGAGTTGTTATATTGATACACTGAATCACATATAACTTCGGTGG
ACGCTGGACAAGGTGGACAGGGTCTTCAGACTGTATCCCTGTACAAAAATCCCGTCCTCA
ATTCCACAAGCCACCTGACGAGGGAAACCTACTTCCGGTCTCGCGGCTGCGAATAATTTT
GAGCTCGGAACACTCTGTTTGTGGCTTGCTGTTTTTCTTTCTCCAGTTGTGGGGGATCTT
TCACAACTTACGATTTTTCTCGCATTTCCAGGGAGCCGGAACAAACCCCTGTTTGTACCG
ACCCCTTTTTCACCTTCCCCTGATGAACGGCTTGTATTCGGAATAAATCCATCTCTGTCG
TGTGATGATGGAACCTCATTTCCTGGAAAATAATTCAGAGTGTTTTGGACGCAAAAAAAA
AAAAAAAAAGCACTTTAAATAGTTTCCTGTGGCTGTTTCAAAAAAAAAAAAAAAAA

Homology of Contig U35_15702 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os07g27790.1	497	protein\|glutamate--cysteine ligase, chloroplast precursor, putative, expressed
Brachypodium v1_2	Bradi2g38340.2	498
Arabidopsis v10	AT4G23100.1	522	\| Symbols: RML1, PAD2, GSH1, CAD2, ATECS1, GSHA \| glutamate-cysteine ligase \| chr4:12103458-12106751 REVERSE LENGTH=522