barSeed - Barley Seed Development Database

Probe CUST_12774_PI390587928

Source Contig U35_12459

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_12774_PI390587928	Hv_44K_v2	TATGGAACAGCAAGCATCATACCTGATCAAGGGATTTTCCTAGCTGCATCAGTTTACCGT	617	GeneList: darkorange2 MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.114	1.026	1.474	1.147	0.700	0.679
Raw	103.577	137.306	188.312	213.403	49.972	51.954

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK358435	3401	2e-24	1793 - 1852	100	60 / 60	plus	ABC13731
MLOC	MLOC_70206.1	396	2e-24	288 - 347	100	60 / 60	plus	None
JLOC1	JLOC1_46581.1	438	8e-25	182 - 241	100	60 / 60	plus
Harvest21	ABC25116	685	3e-25	618 - 677	100	60 / 60	plus
Harvest35	U35_12459	685	3e-25	618 - 677	100	60 / 60	plus

Contig Sequence (685 bp)

>U35_12459 685 Hv_44K_v2
GTATAGATAAAGTTGCAAAAAGAACGGCATCTGTTGTGTCACATTCACATGGAGCTGTCA
CCCGTTACCAACTACCCAAACAGAAAAAGAAGAATGAGGCATCGACATTGGCAACACAAC
TTGTTGCTAGTCCCATCTCTCCAGCTATGGCACCCACAGGAGATGCTCAGCATGCAGCTA
GTCAGCTTCTTGGTTCAATATTTGAGTGTCTAGTAGGCATACTTGAGAACCATATTACCG
TTGGAGAGCTTCTTGAACAGAAATCATCAACTGAAGTTGACAGTGTAAACACACCTCACA
TGGCAAATGGGGACACAAGCTGGAATCCAGATTCTGAATACTCCCAACCCACTGGTGGTT
TTAGTGTCGCATTCTCTCTGTCAGTTGTTCAGAGCGAGTGCCAGCAACTTCTGTGTGAAA
TTTTGCGGGCAACTCCAGAAGCTGCTACTGCTGATGCAGCTGTTCAGACAGCTAGGCTTG
CAAATAAGGACCCAGTGAAGGAAAAGAGGGATGGATCAGAGGGCCTTTCTTTTGCTTTTC
GTGTTACAGATGCAGCAATCTCTGTGCAAAAGGAAGGTCAAGGATGGCGAAGAAACCCAG
CTGTACCACAAGAAGGGTATGGAACAGCAAGCATCATACCTGATCAAGGGATTTTCCTAG
CTGCATCAGTTTACCGTCCTGTCTT

Homology of Contig U35_12459 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os08g22864.2	949	3e-106	protein\|sec8 exocyst complex component specific domain containing protein, expressed
Brachypodium v1_2	Bradi3g20450.1	1053	4e-112
Arabidopsis v10	AT3G10380.1	1053	6e-57	\| Symbols: SEC8, ATSEC8 \| subunit of exocyst complex 8 \| chr3:3219922-3228356 REVERSE LENGTH=1053