barSeed - Barley Seed Development Database

Probe CUST_12573_PI390587928

Source Contig U35_605

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_12573_PI390587928	Hv_44K_v2	GGGATCATGGAATGTCGAGTATTTGCCAATAATCTTCCATATTATTTTTGTGTACGCAAA	1672	GeneList: turquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	1.246	1.093	1.298	0.937	0.300	0.223
Raw	11046.573	15168.820	16050.333	18003.853	2058.940	1658.403

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
JLOC1	JLOC1_53383.2	2631	3e-24	2216 - 2274	100	59 / 59	plus
JLOC1	JLOC1_53383.1	2041	3e-24	1626 - 1684	100	59 / 59	plus
Harvest21	ABC02168	1785	3e-25	1674 - 1733	100	60 / 60	plus	ABC02168
Harvest21	ABC49590	626	2e-22	22 - 79	98	58 / 59	minus
Harvest35	U35_605	1784	3e-25	1673 - 1732	100	60 / 60	plus
Harvest35	U35_47971	626	2e-22	22 - 79	98	58 / 59	minus

Contig Sequence (1784 bp)

>U35_605 1784 Hv_44K_v2
CTCCCTCCCCCTCTCCACTCCCCCCACCCTCCGCTCCCCCGACGCCTCCGCCGCAGCCGC
AATGGACTCCGTCGCGTCGTGGGGGCTGACGCCGCTGGCGGACGCGGACCCGGACGTCTT
CGACCTCATCGAGCGGGAGAAGCGGCGCCAGCGGAGCGGGATCGAGCTCATCGCGTCGGA
GAACTTCACCTCCTTCGCCGTCATCGAGGCGCTCGGCTCGGCCCTCACCAACAAGTACTC
CGAGGGCATGCCGGGGGCCCGCTACTACGGCGGCAACGACGTCATCGACGAGATCGAGAA
CCTCTGCCGCGACCGCGCCCTCGCCGCCTTCCGCCTCGACGCCGCCTCCTGGGGCGTCAA
CGTGCAGCCCTACTCCGGCTCGCCCGCCAACTTCGCCGCCTACACCGCGCTCCTCAACCC
GCACGACCGGATCATGGGGCTCGACCTCCCCTCCGGCGGACACCTCACCCACGGCTACTA
CACCGCCGGGGGGAAGAAGATCTCCGCCACCTCCATCTACTTCGAGAGCCTGCCCTACAA
GGTCAGCGCCGCCAACGGCTACATCGACTACGACAAGCTCGAGGAGAAGGCCATGGACTT
CCGCCCCAAGCTCATCATCTGCGGTGGCAGCGCCTACCCCAGGGACTGGGACTACGCCAA
GCTCAGGGCCATCGCCGACAAGATCGGGGCCATGCTCCTCTGCGACATGGCGCACATCAG
CGGGCTGGTCGCCGCGCAGGAAGCTGCAAATCCTTTTGAGTTCTGTGATGTGGTTACCAC
CACAACACACAAGTCCCTCCGAGGACCAAGGGCTGGCATGATCTTCTACAGGAAAGGCCC
TAAGCCTGCGAAGAAGGGCCAGCCTGAGGGTGCTGTGTATGACTACGAGGACAAGATCAA
CTTTGCGGTGTTCCCATCGCTCCAGGGTGGTCCTCACAACCACCAAATTGCTGCCCTTGC
AGTTGCCCTGAAGCAAACTTTGACACCTGGATTCAAGGCCTACGCAAAGCAGGTCAAGGC
CAACGCTGTTGCCGTTGGAAAGTATCTCATGAGCAAGGGCTACAAGATGGTGACCGATGG
AACTGACAACCACCTTGTTCTATGGGATCTCCGCCCTCTTGGCTTGACTGGAAACAAGGT
CGAAAAGATGTGTGACCTTTGTAGCATTACATTGAACAAGAATGCTGTCTTCGGTGACAG
CAGTGCATTGTCCCCGGGTGGTGTCCGCATTGGTGCTCCTGCTATGACCTCCAGGGGTCT
GGTTGAGAAGGACTTCGAGCAGATCGCCGAGTTCCTCCACCAGGCAGTGACCATCTGCCT
GAACATCCAGAAGGAGCACGGGAAGCTGCTCAAGGACTTCTCCAAGGGCCTGGTGAACAA
CAAGGACATTGAGAACCTCAAGGTCGAGGTCGAGAAGTTTGCCCTCTCCTTTGACATGCC
CGGCTTCACGCTCGAGAGCATGAAGTACAAGGAGTAGGATGATGATACATATGCCATGGT
TGTCTCGCTTCTAAGCCTTGTTGGCCGCGGCGCAATGCCCACCGTGCTGTGCTTCTATGA
GATTTATTGGCTCGCCCTGGAGCCCTGGATAGTTTTGTAAACGCCTCCCACCTCTTGTGC
TCTGTTTGCCGTCCACATCCCCATCATATTTCTTTGTAGGAATTGTTCGCGTGGGATCAT
GGAATGTCGAGTATTTGCCAATAATCTTCCATATTATTTTTGTGTACGCAAAAAAAAAAA
AAAAAAATCTGTGTTGTTTTCCGTTTTAAAAAAAAAAAAAAAAA

Homology of Contig U35_605 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os12g22030.1	532	protein\|serine hydroxymethyltransferase, mitochondrial precursor, putative, expressed
Brachypodium v1_2	Bradi4g19470.1	517
Arabidopsis v10	AT4G13930.1	471	\| Symbols: SHM4 \| serine hydroxymethyltransferase 4 \| chr4:8048013-8050021 REVERSE LENGTH=471