barSeed - Barley Seed Development Database

Probe CUST_12410_PI390587928

Source Contig U35_2124

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_12410_PI390587928	Hv_44K_v2	AAGGATTTGGCATGCCAGTATGCCACTGTTCTAAGCACGTCTAGTTATGAACGGTTTACG	1284	GeneList: yellow MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.650	2.426	1.047	0.873	0.282	0.409
Raw	147.776	205.932	80.499	106.275	11.925	18.827

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	AK362017	1295	2e-24	1232 - 1291	100	60 / 60	plus	ABC07478
MLOC	MLOC_81757.1	5414	2e-24	2732 - 2791	100	60 / 60	minus	ABC07478 ABC21050
JLOC1	JLOC1_54386.1	1836	8e-25	1019 - 1078	100	60 / 60	plus
JLOC1	JLOC1_54385.2	3177	8e-25	2390 - 2449	100	60 / 60	minus
JLOC1	JLOC1_54385.1	3516	8e-25	2729 - 2788	100	60 / 60	minus
Harvest21	ABC07478	1201	3e-25	4 - 63	100	60 / 60	minus	ABC07478
Harvest35	U35_2124	1347	3e-25	1285 - 1344	100	60 / 60	plus

Contig Sequence (1347 bp)

>U35_2124 1347 Hv_44K_v2
CTGTGTATGTACTACGCTCGGTTCTGAAACCACGACCCACCCAGCAGCCAGAGGGAAAGG
GGGAGAAGGAGACACCGCGGCGGGCGGAGAGGCAATGGCGACGACGACGTGCCTGCTGGC
GGCCTGGGCGCTGGTCGCCGCCGGACTGTTCGGCACGGGCCTCGCCCGGGCGTCCTTCGC
CCCGGCCGCCGTCGGGAAGGAGCAGCGCGAGTTCGACTACTTCGCGCTGGCGCTGCAGTG
GCCGGGCACCATCTGCTCCTCCACCCGCCACTGCTGCGCCGTCAACGGCTGCTGCCGGAA
GGAGCCGCTCCACACGTTCACGATCCACGGGCTGTGGCCGGACTACGATGACGGGACTTG
GCCGTCGTGCTGCCGCCACACCAGTTTCGACATGGACAAGATAACGCCTTTGAAGCCCAC
TCTGGACAAGTACTGGCCGTCCCTCTACTGTTCGTCCTCTTCCACTTGTTTCAGTGGTAG
GGGCCCCTTCTGGGCTCATGAGTGGGAGAAGCATGGAACATGCTCGGCCCCTGCTGTTCG
GGAAGAATTACAGTACTTCAGCACTGCCCTTGATCTCTACTTCAAATATAACGTGACAGA
AATGTTGGCGACTGGAGATATCCTGGTCTCCAACGGTAAAGAATATGCACTGAGTGATGT
CATTGACACCATCAAGCATGCTTTTGGGGGCTCACCGCAGATCATCTGCAAGAAGGGCTC
AGTTCAAGAACTAAGGTTATGCTTCACCAAAGATTTGAAGCCTCGTGATTGCCTTACTAC
TTCGGCAATGTACAAGAACCTATCGAAATCAAAGCATTGCCCTCGAAAAATCTCCCTGCC
AACATATGATCCCATCGTACTCGCCAATTCAACACTGGAGATTGTCCCAGAAGCCAGCGA
CAATGGGCTGTACTTCTTTACATCCTAAGATACTGAGCATTGCTGGGTTACGAGGAAACA
GATGCCTTCACGGCCCCGACTGCTTGCAAGACCTGTAGCCACAGATGCTGGGCACTAAGA
TCCCAGTAAAATGGGTCTCTGGTTTGTCCATGCTATTTGTAAAGGAGAACATTCCACATT
AGAACAACTTAGTTCAATTATGTTCTGAATATTTATAAAACTGCTGGAAATCAAATACTC
AGCTCAGATGCCTCGTTGTATGAATAATGAAAGTTGCATGTACATACTAAAAAAAAAAAA
AACACGCTTTAGCTGGGCAAGGGCCCTTTGTACTTCATTGGATCTTTAGCTGGGCAGGGC
CCTTTGTACTTCTTTGGATTACCAAAGGATTTGGCATGCCAGTATGCCACTGTTCTAAGC
ACGTCTAGTTATGAACGGTTTACGCTG

Homology of Contig U35_2124 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os01g67180.1	285	7e-143	protein\|ribonuclease T2 family domain containing protein, expressed
Brachypodium v1_2	Bradi2g57620.1	285	1e-88
Arabidopsis v10	AT2G39780.1	259	1e-80	\| Symbols: RNS2 \| ribonuclease 2 \| chr2:16591256-16593515 FORWARD LENGTH=259