barSeed - Barley Seed Development Database

Probe CUST_11662_PI390587928

Source Contig U35_2608

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_11662_PI390587928	Hv_44K_v2	TTTCTACAGTCTGTACATGAGACAGGACAGGGTCAATTGATAGTTTTGACATTTCTACAG	2141	GeneList: grey60 MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	6.057	5.042	1.469	0.535	0.298	0.303
Raw	157.613	217.995	53.896	33.428	5.618	6.545

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_4380.1	3389	2e-23	62 - 119	100	58 / 58	minus	ABC08402
MLOC	MLOC_4380.2	3390	2e-23	62 - 119	100	58 / 58	minus	ABC08402
MLOC	MLOC_4380.3	3751	2e-23	62 - 119	100	58 / 58	minus	ABC08402
JLOC1	JLOC1_2714.1	3428	1e-23	3201 - 3258	100	58 / 58	plus
Harvest35	U35_2608	2237	3e-25	2142 - 2201	100	60 / 60	plus

Contig Sequence (2237 bp)

>U35_2608 2237 Hv_44K_v2
GGGAAGGTCACGCTCTACCAGGACGCGCACGTCAACGAGGGGGACCTGCCGGACGTCCAG
CTCGAGCGGGGGAAGAAGTTCGAGCACAACCAGTGCTGGGAGGACATCTGCCACGCCATA
CTCGAGGCGCACCATATGATTTACATCGTCGGCTGGTCGATCTACGACAAGGTGAAGCTG
GTGCGCGAGCCGTCCTCGTCCAGGCCGCTCCCAGAGGGCGGCAACCTCACGCTCGGAGAC
CTGCTCAAGTTCAAGTCGCAGGAGGGCGTCAGAGTATGCCTGCTCGTCTGGGACGACAAG
ACGTCCCATGACAAGCTCTTCATCAAAACGGGTGGTGTCATGGGCACACATGATGGAGAA
ACTAGAAAGTTTTTCAAGCATTCCTCAGTCATCTGTGTTCTTTCACCACGATATGCCAGC
AGTAAGCTGAGCATTTTCAAACAACAGGTTGTTGGGACATTGTTTACTCACCACCAAAAA
TGTGTGCTGGTTGATACACAAGCTTCGGGAAACAAGAGGAAAGTTACTGCTTTCGTTGGG
GGCCTAGATCTTTGTGATGGGCGTTACGATACTCCTCAACATAGGCTTTTTAAAGACCTT
AACACCGTATTTGAAAATGATTTCCACAACCCAACATTTTCGGCAGGCACAAAAGGACCA
AGACAACCTTGGCATGATTTGCACTGTAAGATTGATGGACCAGCTGCATATGATGTCTTG
AAGAATTTTGAACAACGCTGGAGAAAGGCATCCAAATTCCGTGATCGATTTAGGAAAGTA
TCTCGCTGGAAAGATGATGCCTTGATAAAGCTGGAGCGTATCTCGTGGATACTTAGTCCT
TCACCTAATGTTCCAAATGATCATGTTAGTTTGCGGGTTTCAAAAGAAGAAGATCCTGAA
AATTGGCATGTTCAGGTATTCCGGTCAATTGACTCGGGCTCTCTAAAAGGATTTCCTAGT
GATTGCAAAGAGGCTTCAAAGCAGAATCTTGTGTGCCGAAAGAACCTCATAATTGATAAG
AGCATTCACACAGCATATGTACGGGCAATCAGATCGGCACAACATTTCATTTATATAGAA
AATCAGTATTTTCTTGGTTCTTCGTATGAGTGGCCATCCTATGTGAATTCAGGTGCTGAT
AATCTGATACCAATGGAATTGGCCCTCAAAATTGCAACCAAGATTAGAGCTGGCGAACGC
TTTGCAGTTTATGTAGTCATACCAATGTGGCCTGAAGGAGTCCCTACCTCGGCCTCTGTG
CAAGAAATTCTTTTCTTTCAGGCTCAGACAATGGAGATGATGTATGGAGTAATCGCACGA
GAGCTCAAGGCCATGAACATTGAGAATGCACACCTTCAGGATTACTTGAACTTTTACTGT
CTTGGCAATCGGGAAGAACCGACAACAGATGGTAGCCCCGAGTCAGATAAATCTACAGAT
AAAAGTGCAGCGGCCCTGGCAAGAAAACATCGGCGGTTCATGATCTATGTTCATGCAAAA
GGGATGATTGTAGACGATGAATACGTCATTTTGGGGTCAGCAAACATAAATCAGAGATCC
TTGGCTGGTTCGAGAGATACTGAAATCGCAATGGGTGCCTACCAGCCTCATCATGCATGG
TCTTCGAAGAAGGGTCATCCTCACGGCCAGGTATATGGGTATAGGAACTCCCTCTGGGCT
GAACACCTTGGTATGGTTGATGAGCGCTTCAAGGAACCTTCTAGTCTGGAGTGTGTACGA
TTAGTGAACCAAATAGCTGAAGATAACTGGGAAAGATTCGCATCAGAAGAGATGAAAATG
TTGCAAGGGCACCTCCTCAGATACCCAGTGAAGGTAGAGCCTGATGGTAAGATCGTCCCG
CTGCCCGACCAAGAATGCTTCCCCGACGTCGGTGGCAAGATCTGCGGCGCCCCGACCTCG
CTTCCTGATTCGCTGACGATGTGACGTCACGGTACTGACTTCTCTTCAGAGTCCAAGCTG
GTTATTTTGCTCTCCTGTAAAACCTCTCTGAATGTCTTGTCTTGCCAACGGATGATGATA
ACCTGCACCGTAATCTGTGGATCACACATTGTGTGGCGTATAGGGAAAGCACGGGCTGTT
CACGGTGTGTTGTAGAGTTGTGCGCCTTTGTAAGATTTTTTTTTCTACAGTCTGTACATG
AGACAGGACAGGGTCAATTGATAGTTTTGACATTTCTACAGAAAAAAAAAAAAATACAAA
AAAAAAAAAAAAAAAAG

Homology of Contig U35_2608 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	Description
Rice v7	LOC_Os09g37100.1	855	protein\|phospholipase D, putative, expressed
Brachypodium v1_2	Bradi4g36800.1	859
Arabidopsis v10	AT4G35790.1	868	\| Symbols: ATPLDDELTA, PLDDELTA \| phospholipase D delta \| chr4:16955774-16959875 REVERSE LENGTH=868