barSeed - Barley Seed Development Database

Probe CUST_11483_PI390587928

Source Contig U35_40389

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_11483_PI390587928	Hv_44K_v2	TTGATAGGACTGATATACGAGGGGTAAATGTAGGCATGGATCACTTCTTTTACTGCTTGT	3	GeneList: paleturquoise MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.887	1.805	1.125	0.918	0.422	0.293
Raw	147.542	159.225	83.174	109.137	16.611	13.067

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_21289.1	1553	2e-24	1355 - 1414	100	60 / 60	plus	None
JLOC1	JLOC1_14777.1	1911	8e-25	1628 - 1687	100	60 / 60	plus
Harvest35	U35_40389	651	3e-25	4 - 63	100	60 / 60	minus

Contig Sequence (651 bp)

>U35_40389 651 Hv_44K_v2
TTTACAAGCAGTAAAAGAAGTGATCCATGCCTACATTTACCCCTCGTATATCAGTCCTAT
CAACTAATCACCATTCAAGTCAGGTTTACGACCAGCCAAACTTGTAGTCGAGGATGTAAA
CCAAGATCAGCGACAGCGGGTACAGAAGGAATGCGACGAAGCACGTCCACAGGGGGAACA
TGGTCCGGAAGCTGGTGAAGAGTCCCATTATGACACAGACGAGGAAGATGACGAGGACCT
CCGATGAGAAGTCCCAAGTTAAGCCCCTGATGTAGACGAGAGCCAGGAACACGGCCAGGC
AGAGCGTGTTGTTCATGGTCACTCCACCGTACACCTCTGAGAATGTCAGAGACAGAGTGC
GTTGCTTCTTTCGGCTGGCAAAGATAATTGCTGAGACAGCCTCACTGGAATTGGTAGCCA
AGGGCATCGCAATGAAGGAAATGAAAAAAGACGGTATACTGGTAGCACTTGAAAAGTTGT
GCACAGCATCAACAAGTGGGTCTGCAAATGCAGCTGCCATTGCAGTTCCGAGAAGCAAAA
GTGAAATAGCCTTGAAGCATGTCCAGGTTGGATTTTCAATAGATTCATCATCCTCTTCAT
GCTTGTCAAGCAGTGCATTAATGCTCTTCCCCTGTTGTAATGTGAAAATCA

Homology of Contig U35_40389 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os02g14980.2	427	8e-105	protein\|calcium-binding EF hand family protein, putative, expressed
Brachypodium v1_2	Bradi3g09350.1	579	5e-109
Arabidopsis v10	AT1G53210.1	585	5e-83	sodium/calcium exchanger family protein / calcium-binding EF hand family protein \| chr1:19844790-19847533 FORWARD LENGTH=585