barSeed - Barley Seed Development Database

Probe CUST_10629_PI390587928

Source Contig U35_46

barSeed

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Probe Details

Probe ID	Chip	Probe Sequence (60 bp)	Probe location	Differentially Expressed
CUST_10629_PI390587928	Hv_44K_v2	GTGCTTGTCTGGTGTTAGTGAGATCTGCTTGAGTTGATATGTATTATTTCGTTCAAACGC	1203	GeneList: blue MasterList: None

Probe Values

	3 dpa	4 dpa	5 dpa	6 dpa	7 dpa	8 dpa
Normalized	2.007	1.356	1.029	0.900	0.307	0.310
Raw	430.872	449.441	300.530	426.101	51.247	55.350

Probe Sequence's BLAST hits in Barley Unigene Datasets

Dataset	Hit	Hit Length	E-value	Position on Hit	% Identity	Match	Direction	Probes on Sequence
MLOC	MLOC_5958.2	2308	2e-24	1089 - 1148	100	60 / 60	minus	ABC05782 ABC06216 ABC07052
JLOC1	JLOC1_3870.1	2648	8e-25	1262 - 1321	100	60 / 60	plus
Harvest21	ABC06216	1235	3e-25	1152 - 1211	100	60 / 60	plus	ABC06216
Harvest35	U35_46	1287	3e-25	1204 - 1263	100	60 / 60	plus

Contig Sequence (1287 bp)

>U35_46 1287 Hv_44K_v2
GGGTGGTTATAGTGTTTAAGTGACACAATGTGACCCTGCTTTTTCAGTGGCAAAAGAAGT
GGGAGAGAGAAAGAGCTCTGGACGACTGAAACCCTACCCGGAGCCCCGCCCCCGCCGCCG
TCGCCTCTCCCTCTCGCTGTTTAGAGCCGTCGCGCCGCCAGCAGAAGAGGGGGTGAAGAA
GGCCAGCGTCACCATGGCGGACGAGGAGCAGATGGAGCGGAAGGAGGAGGCCACCGAGAT
TGCACCTTTTGATCCAACCAAGAAGAAGAAGAAGAAGAAGGTCGTCATCCAAGATCCAGC
TGATGAGGTTGATAAGCTGGCAGAGAAGACAGAGGGCTTGTCAGTCACTGAATCTGGCGA
GCCAAGCTTCGCGGGATTGAAAAAGAAAAAGAAGAAAGTTGTGGAACTTGATCCATCGCT
TGTTGAGGCTGGAGATGGTGAAGACACTCTTGATGATCAAGTCGGAGAGGATGAACAAGG
AGAAGGGATTGTGCTGGGTGGAGCAACCCAATACCCATGGGAAGGAACTGATAGAGATTA
CAAATACGATGAGCTGCTTGGCAGAGTCTTCAATATCCTGCGTGAGAATAATCCAGATCT
TGCTGGTGATAGACGAAGAACAGTTATGCGGCCTCCTCAAGTTCTTAGGGAAGGCACAAA
GAAGACAGTTTTTGTGAACTTCATGGACTTGTGCAAAACAATGCATAGGCAACCTGAACA
TGTGATGATGTTTTTACTTGCTGAGATGGGAACAAGTGGATCCCTTGATGGGCAACAAAG
GTTAGTTATCAAAGGAAGATTTGCCCCGAAGAACTTTGAAGCTATACTCAGGAGATACAT
CAATGAATATGTCATATGTAATGGATGTAAGAGCCCAGATACCATCCTTTCCAAGGAGAA
TCGGTTGTTCTTCCTTCGTTGTGAACAATGTGGTTCGTCAAGGTCAGTTGCTCCTATCAA
GGCTGGATTTGTGGCGCAAGTTGGGCGTCGGAAGGCTGGAACTTAAGCTGCTCTGTCAAC
GTAATCCAACAATTTGCCGATTTACAAACAATCTTTATTGTCCAGCTTATACTGTGCCTA
GACCGCCTTGTGGTTTTTGTTATTTCGTGTACTATGAATATGAATGCTGCTGCTGCTGCT
GTGAATTTCTATTTATCTCAGAGGATCTAAGTTTGCTGCCTATTGCTGGCTCCTTTGCCT
TTTGTGCTTGTCTGGTGTTAGTGAGATCTGCTTGAGTTGATATGTATTATTTCGTTCAAA
CGCNCCCAGTAAAAAAAAAAAAAAAAA

Homology of Contig U35_46 to Model Species (E-value threshold 1e-50)

Pseudo-peptide dataset	Hit	Hit length	E-value	Description
Rice v7	LOC_Os03g21550.2	271	6e-169	protein\|eukaryotic translation initiation factor 2 subunit beta, putative, expressed
Brachypodium v1_2	Bradi1g63310.1	271	2e-173
Arabidopsis v10	AT5G20920.1	268	5e-117	\| Symbols: EIF2 BETA, EMB1401 \| eukaryotic translation initiation factor 2 beta subunit \| chr5:7094994-7096661 REVERSE LENGTH=268